Conantokin-Rl2[K8N,A9Q,del10O] (P05848) Protein Card

General Information

Name	Conantokin-Rl2[K8N,A9Q,del10O]
Alternative name(s)	conRl-B[desKAO;N8Q9]
Organism	synthetic construct
Protein Type	Synthetic
Parent	Conantokin-Rl2

Classification

Conopeptide class	conantokin
Gene superfamily
Pharmacological family

Sequence

GE(Gla)(Gla)LA(Gla)NQ(Gla)FAR(Gla)LAN(nh2)
Modified residues	position symbol name 3 Gla Gamma carboxylic glutamic acid 4 Gla Gamma carboxylic glutamic acid 7 Gla Gamma carboxylic glutamic acid 10 Gla Gamma carboxylic glutamic acid 14 Gla Gamma carboxylic glutamic acid 18 nh2 C-term amidation
Average Mass	2168.08
Monoisotopic Mass	2166.84
Isoelectric Point	1.61
Extinction Coefficient [280nm]	NA

Activity

IC50: Ionotropic glutamate receptors

Target	Organism	IC50		Agonist	Ref
GluN2A	R. norvegicus	>10uM		200uM glutamate	Gowd,K.H. et al. (2012)
GluN2B	R. norvegicus	>10uM		200uM glutamate	Gowd,K.H. et al. (2012)
GluN2C	R. norvegicus	>10uM		200uM glutamate	Gowd,K.H. et al. (2012)
GluN2D	R. norvegicus	>10uM		200uM glutamate	Gowd,K.H. et al. (2012)

References

Gowd,K.H., Han,T.S., Twede,V., Gajewiak,J., Smith,M.D., Watkins,M., Platt,R.J., Toledo,G., White,H.S., Olivera,B.M. and Bulaj,G. (2012) Conantokins derived from the Asprella clade impart conRl-B, an N-methyl d-aspartate receptor antagonist with a unique selectivity profile for NR2B subunits. Biochemistry 51:4685-4692

Kunda,S., Yuan,Y., Balsara,R.D., Zajicek,J. and Castellino,F.J. (2015) Hydroxyproline-induced Helical Disruption in Conantokin Rl-B Affects Subunit-selective Antagonistic Activities toward Ion Channels of N-Methyl-d-aspartate Receptors. J. Biol. Chem. 290:18156-18172

Internal links

Nucleic acids
Structure	The Solution Structure of the Magnesium-bound Conantokin-R1B [K8N,A9Q,del10O]

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 5 December 2023