LvIA (P05949) Protein Card

General Information

Name	LvIA
Organism	Conus lividus
Organism region	Indo-Pacific
Organism diet	vermivorous
Protein Type	Wild type
Protein precursor	LvIA precursor (5886)
Notes	Zhu, X et al., 2016 reported recombinantly-expressed LvIA used in a hot-plate pain model produced increased latency which was maximal 90 min after treatment (24.17 s reaction time). Kondasinghe,T.D et al.,2019 reported three on-resin strategies to efficiently synthesize LvIA.

Classification

Sequence

GCCSHPACNVDHPEIC(nh2)

Modified residues

position	symbol	name
17	nh2	C-term amidation

Sequence evidence

nucleic acid level

Average Mass

1679.87

Monoisotopic Mass

1678.61

Isoelectric Point

5.63

Extinction Coefficient [280nm]

-1.00

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism		IC50		n_hill	Competitor	Agonist	Ref
α3β2	H. sapiens	(Recombinant peptide against Xenopus oocyte expressing human nAChR subtype.)	46.8 nM				ACh (100 uM)	Zhu,X. et al. (2016)
	R. norvegicus	(Recombinant peptide against Xenopus oocyte expressing rat nAChR subtype.)	160.8 nM				ACh (100 uM)	Zhu,X. et al. (2016)
			15.6 nM	[12.8-19.1]	0.71			Zhu,X. et al. (2020)
α3β4	R. norvegicus		283 nM	[237-338]	1.47			Zhu,X. et al. (2020)
AChBP	A. californica		2300 nM	+/-200		α-bungarotoxin (0.9 nM)		Zhu,X. et al. (2020)

Synthetic variants

References

Luo,S., Zhangsun,D., Schroeder,C.I., Zhu,X., Hu,Y., Wu,Y., Weltzin,M.M., Eberhard,S., Kaas,Q., Craik,D.J., McIntosh,J.M. and Whiteaker,P. (2014) A novel α4/7-conotoxin LvIA from Conus lividus that selectively blocks α3β2 vs. α6/α3β2β3 nicotinic acetylcholine receptors. FASEB J. 28:1842-1853

Zhu,X., Bi,J., Yu,J., Li,X., Zhang,Y., Zhangsun,D. and Luo,S. (2016) Recombinant Expression and Characterization of α-Conotoxin LvIA in Escherichia coli. Mar Drugs 14

Kondasinghe, T.D., Saraha, H.Y., Jackowski, S.T. and Stockdill, J.L. (2019) Raising the bar on-bead: Efficient on-resin synthesis of α-conotoxin LvIA Tetrahedron letters 60:23-28

Zhu,X., Pan,S., Xu,M., Zhang,L., Yu,J., Yu,J., Wu,Y., Fan,Y., Li,H., Kasheverov,I.E., Kudryavtsev,D.S., Tsetlin,V.I., Xue,Y., Zhangsun,D., Wang,X. and Luo,S. (2020) High Selectivity of an α-Conotoxin LvIA Analogue for α3β2 Nicotinic Acetylcholine Receptors Is Mediated by β2 Functionally Important Residues. J Med Chem 63:13656-13668

Internal links

Protein Precursor	LvIA precursor (5886)
Nucleic acids
Structure	A novel Î±4/7-conotoxin LvIA from Conus lividus that selectively blocks Î±3Î²2 vs. Î±6/Î±3Î²2Î²3 nicotinic acetylcholine receptors Co-crystal structure of Ac-AChBPP in complex with alpha-conotoxin LvIA

External links

Ncbi

2MDQ_A

Tools