PIA (P00595) Protein Card

General Information

Name	PIA
Alternative name(s)	P1a
Organism	Conus purpurascens
Organism region	Eastern Pacific
Organism diet	piscivorous
Protein Type	Wild type
Protein precursor	PIA precursor (410) PIA precursor (3011)

Classification

Conopeptide class	conotoxin
Gene superfamily	A superfamily
Cysteine framework	I
Pharmacological family	alpha conotoxin

Sequence

RDPCCSNPVCTVHNPQIC(nh2)

Modified residues

position	symbol	name
19	nh2	C-term amidation

Sequence evidence

nucleic acid level

Average Mass

1981.26

Monoisotopic Mass

1979.82

Isoelectric Point

7.88

Extinction Coefficient [280nm]

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		n_hill	Agonist	Ref
α1β1δε	H. sapiens	>10uM			10uM ACh	Dowell,C. et al. (2003)
α2β2	R. norvegicus	>10uM			100uM ACh	Dowell,C. et al. (2003)
α2β4	R. norvegicus	>10uM			100uM ACh	Dowell,C. et al. (2003)
α3β2	R. norvegicus	74nM	+/-36	0.8	100uM ACh	Dowell,C. et al. (2003)
α3β4	R. norvegicus	518nM	+/-200	0.93	100uM ACh	Dowell,C. et al. (2003)
α4β2	R. norvegicus	>10uM			100uM ACh	Dowell,C. et al. (2003)
α4β4	R. norvegicus	>10uM			100uM ACh	Dowell,C. et al. (2003)
α6β4	R. norvegicus	33nM	+/-16	1.1	100uM ACh	Dowell,C. et al. (2003)
α6/α3β2	R. norvegicus	690pM	+/-300	0.81	100uM ACh	Dowell,C. et al. (2003)
α6/α3β2β3	H. sapiens	1.7nM	+/-1	0.98	100uM ACh	Dowell,C. et al. (2003)
	R. norvegicus	950pM	+/-350	1.1	100uM ACh	Dowell,C. et al. (2003)
α6/α3β4	H. sapiens	12.6nM	+/-6	1.2	100uM ACh	Dowell,C. et al. (2003)
	R. norvegicus	30nM	+/-17	0.93	100uM ACh	Dowell,C. et al. (2003)

Ki: Nicotinic acetylcholine receptors

Target	Organism		Ki		Competitor	Agonist	Ref
a7α6β2	R. norvegicus	( 0.1nM epibatidine)	35uM			0.1nM epibatidine	Pucci,L. et al. (2011)
		(0.1nM epibatidine)	32nM			0.1nM epibatidine	Pucci,L. et al. (2011)

Synthetic variants

PIA Î”1	DPCCSNPVCTVHNPQIC(nh2)
PIA Î”1-2	PCCSNPVCTVHNPQIC(nh2)
PIA Î”1-3	CCSNPVCTVHNPQIC(nh2)
PIA [R1ADMA]	(ADMA)DPCCSNPVCTVHNPQIC(nh2)
PIA [R1E]	EDPCCSNPVCTVHNPQIC(nh2)
PIA [R1K]	KDPCCSNPVCTVHNPQIC(nh2)
PIA [R1L]	LDPCCSNPVCTVHNPQIC(nh2)

References

Dowell,C., Olivera,B.M., Garrett,J.E., Staheli,S.T., Watkins,M., Kuryatov,A., Yoshikami,D., Lindstrom,J.M. and McIntosh,J.M. (2003) Alpha-conotoxin PIA is selective for alpha6 subunit-containing nicotinic acetylcholine receptors J. Neurosci. 23:8445-8452

Chi,S.W., Lee,S.H., Kim,D.H., Kim,J.S., Olivera,B.M., McIntosh,J.M. and Han,K.H. (2005) Solution structure of alpha-conotoxin PIA, a novel antagonist of alpha6 subunit containing nicotinic acetylcholine receptors Biochem. Biophys. Res. Commun. 338:1990-1997

Pucci,L., Grazioso,G., Dallanoce,C., Rizzi,L., De Micheli,C., Clementi,F., Bertrand,S., Bertrand,D., Longhi,R., De Amici,M. and Gotti,C. (2011) Engineering of α-conotoxin MII-derived peptides with increased selectivity for native α6β2* nicotinic acetylcholine receptors. FASEB J. 25:3775-3789

Himaya, S.W.A., Mari, F. and Lewis, R.J. (2018) Accelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals Scientific reports 8:1-8

Internal links

Protein Precursor	PIA precursor (410) PIA precursor (3011)
Nucleic acids
Structure	SOLUTION CONFORMATION OF ALPHA-CONOTOXIN PIA

External links

Ncbi

1ZLC_A

Tools