PeIA[S9R] (P06794) Protein Card

General Information
Name PeIA[S9R]
Alternative name(s) [Arg9]PeIA
Organism synthetic construct
Protein Type Synthetic
Parent PeIA

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSHPACRVNHPELC(nh2)
Modified residues
positionsymbolname
17nh2C-term amidation
Sequence evidence Unknown
Average Mass 1720.97
Monoisotopic Mass 1719.68
Isoelectric Point 8.42
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α3β2R. norvegicus4.1 nM+/-0.7100 uM AchHone,A.J. et al. (2013)
α3β4R. norvegicus18.7 μM[5.09-68.9]ACh(300 μM)Hone,A.J. et al. (2020)
α6β4R. norvegicus4.5 uM+/-1.4100 uM AchHone,A.J. et al. (2013)
α6/α3β2β3R. norvegicus2.29 nM+/-0.7100 uM AchHone,A.J. et al. (2013)
α6/α3β4H. sapiens96.8 nM[86.2-109]300 uM AChHone,A.J. et al. (2021)
R. norvegicus9750 nM[8522-11150]300 uM AChHone,A.J. et al. (2021)

References
Hone,A.J., Ruiz,M., Scadden,M., Christensen,S., Gajewiak,J., Azam,L. and McIntosh,J.M. (2013) Positional scanning mutagenesis of α-conotoxin PeIA identifies critical residues that confer potency and selectivity for α6/α3β2β3 and α3β2 nicotinic acetylcholine receptors. J. Biol. Chem. 288:25428-25439
Hone,A.J., Rueda-Ruzafa,L., Gordon,T.J., Gajewiak,J., Christensen,S., Dyhring,T., Albillos,A. and McIntosh,J.M. (2020) Expression of α3β2β4 nicotinic acetylcholine receptors by rat adrenal chromaffin cells determined using novel conopeptide antagonists J Neurochem
Hone,A.J., Kaas,Q., Kearns,I., Hararah,F., Gajewiak,J., Christensen,S., Craik,D.J. and McIntosh,J.M. (2021) Computational and Functional Mapping of Human and Rat α6β4 Nicotinic Acetylcholine Receptors Reveals Species-Specific Ligand-Binding Motifs. J Med Chem 64:1685-1700

Internal links
Nucleic acids
Structure

External links

Tools