PeIA[A7V,S9H,V10A,N11R,E14A] (P06804) Protein Card

General Information

Name	PeIA[A7V,S9H,V10A,N11R,E14A]
Alternative name(s)	PeIA-5069
Organism	synthetic construct
Protein Type	Synthetic
Parent	PeIA

Classification

Conopeptide class	conotoxin
Gene superfamily
Cysteine framework	I
Pharmacological family	alpha conotoxin

Sequence

GCCSHPVCHARHPALC(nh2)

Modified residues

position	symbol	name
17	nh2	C-term amidation

Sequence evidence

Unknown

Average Mass

1685.97

Monoisotopic Mass

1684.69

Isoelectric Point

11.16

Extinction Coefficient [280nm]

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism		IC50		Agonist	Ref
α3β2	R. norvegicus	(a3 mutant [K152E)	18.2 nM	+/-3.2	100 uM Ach	Hone,A.J. et al. (2013)
		(E184D)	30.9 uM	+/-9.7	100 uM Ach	Hone,A.J. et al. (2013)
α3β4	R. norvegicus	(Q195T] )	> 10 uM		100 uM Ach	Hone,A.J. et al. (2013)
α4β2	R. norvegicus		> 10 uM		100 uM Ach	Hone,A.J. et al. (2013)
α4β4	R. norvegicus		> 10 uM		100 uM Ach	Hone,A.J. et al. (2013)
α6β4	R. norvegicus		43.8 nM	+/-6.9	100 uM Ach	Hone,A.J. et al. (2013)
α6/α3β2β3	R. norvegicus		2.16 nM	+/-0.18	100 uM Ach	Hone,A.J. et al. (2013)
			311 nM	+/-117	100 uM Ach	Hone,A.J. et al. (2013)
			5.5 uM	+/-3.1	100 uM Ach	Hone,A.J. et al. (2013)
α7	R. norvegicus		> 10 uM		200 uM Ach	Hone,A.J. et al. (2013)

References

Hone,A.J., Ruiz,M., Scadden,M., Christensen,S., Gajewiak,J., Azam,L. and McIntosh,J.M. (2013) Positional scanning mutagenesis of α-conotoxin PeIA identifies critical residues that confer potency and selectivity for α6/α3β2β3 and α3β2 nicotinic acetylcholine receptors. J. Biol. Chem. 288:25428-25439

Hone,A.J., Kaas,Q., Kearns,I., Hararah,F., Gajewiak,J., Christensen,S., Craik,D.J. and McIntosh,J.M. (2021) Computational and Functional Mapping of Human and Rat α6β4 Nicotinic Acetylcholine Receptors Reveals Species-Specific Ligand-Binding Motifs. J Med Chem 64:1685-1700

Internal links

Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 28 March 2023