GVIIJ[(Btr)2W,(Scc)24C] (P06833) Protein Card

General Information

Name	GVIIJ[(Btr)2W,(Scc)24C] (named by ConoServer)
Alternative name(s)	GVIIJ[SH],GVIIJ[SSC]
Organism	synthetic construct
Protein Type	Synthetic
Parent	GVIIJ
Notes	Block of rNav1.2[C912A] or [C918A] mutants is slowly reversible. +DTT it is rapidly reversible [Zhang,M.M., Gajewiak (2015)].

Classification

Conopeptide class	conotoxin
Gene superfamily
Cysteine framework	VI/VII
Pharmacological family	mu conotoxin

Sequence

GWCGDOGATCGKLRLYCCSGFCDCYTKTCKDKSSA

Modified residues

position	symbol	name
6	O	4-Hydroxyproline

Sequence evidence

protein level

Average Mass

3739.22

Monoisotopic Mass

3736.50

Isoelectric Point

10.10

Extinction Coefficient [280nm]

8605.00

Activity

IC50: Voltage-Gated Sodium Channels

Target	Organism		IC50		Ref
Na_v1.1	H. sapiens	(Expressed in CHO cells)	23 nM	+/-10	Gajewiak,J. et al. (2014)
Na_v1.2	H. sapiens	(Expressed in HEK 293 cells)	55 nM	+/-23	Gajewiak,J. et al. (2014)
Na_v1.3	H. sapiens	(Expressed in CHO cells)	139 nM	+/-38	Gajewiak,J. et al. (2014)
Na_v1.4	H. sapiens	(Expressed in HEK 293 cells)	14 nM	+/-4	Gajewiak,J. et al. (2014)
Na_v1.6	H. sapiens	(Expressed in CHO cells)	14 nM	+/-11	Gajewiak,J. et al. (2014)
Na_v1.7	H. sapiens	(Expressed in HEK 293 cells)	78 nM	+/-21	Gajewiak,J. et al. (2014)

Percentage inhibition: Voltage-Gated Sodium Channels

Target	Organism		% inhibition		Concentration	Ref
Na_v1.2	R. norvegicus	(Expressed in X. laevis oocytes. )	88	+/-4	10 Î¼M	Zhang,M.M. et al. (2015)
		(Mutant: Nav1.2[C918A]. Expressed in X. laevis oocytes.)	88	+/-2	10 Î¼M	Zhang,M.M. et al. (2015)
		(Mutant: Nav1.2[C912A]. Expressed in X. laevis oocytes.)	92	+/-4	10 Î¼M	Zhang,M.M. et al. (2015)
Na_v1.5	H. sapiens	(Expressed in HEK 293 cells)	0	+/-6	10 Î¼M	Gajewiak,J. et al. (2014)

References

Gajewiak,J., Azam,L., Imperial,J., Walewska,A., Green,B.R., Bandyopadhyay,P.K., Raghuraman,S., Ueberheide,B., Bern,M., Zhou,H.M., Minassian,N.A., Hagan,R.H., Flinspach,M., Liu,Y., Bulaj,G., Wickenden,A.D., Olivera,B.M., Yoshikami,D. and Zhang,M.M. (2014) A disulfide tether stabilizes the block of sodium channels by the conotoxin μO§-GVIIJ. Proc. Natl. Acad. Sci. U.S.A. 111:2758-2763

Zhang,M.M., Gajewiak,J., Azam,L., Bulaj,G., Olivera,B.M. and Yoshikami,D. (2015) Probing the Redox States of Sodium Channel Cysteines at the Binding Site of μO§-Conotoxin GVIIJ. Biochemistry 54:3911-3920

Green,B.R., Gajewiak,J., Chhabra,S., Skalicky,J.J., Zhang,M.M., Rivier,J.E., Bulaj,G., Olivera,B.M., Yoshikami,D. and Norton,R.S. (2016) Structural Basis for the Inhibition of Voltage-gated Sodium Channels by Conotoxin μO§-GVIIJ. J. Biol. Chem. 291:7205-7220

Internal links

Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 21 March 2023