GVIIJ[(Btr)2W] (P06835) Protein Card

General Information

Name	GVIIJ[(Btr)2W] (named by ConoServer)
Alternative name(s)	GVIIJ[SSC]
Organism
Protein Type	Synthetic
Parent	GVIIJ
Notes	No activity against mouse Nav 1.6 if co-expressed with β2 or β4 subunit [Wilson, M.J., Zhang, M. (2015)]. Does not block A-fiber or C-fiber compound action potentials in mouse and rat sciatic nerves [Wilson, M.J., Zhang, M. (2015)].

Classification

Conopeptide class	conotoxin
Gene superfamily	O1 superfamily
Cysteine framework	VI/VII
Pharmacological family	mu conotoxin

Sequence

GWCGDOGATCGKLRLYCCSGFCD(Scc)YTKTCKDKSSA

Modified residues

position	symbol	name
6	O	4-Hydroxyproline
24	Scc	S-cysteinylated cysteine

Sequence evidence

protein level

Average Mass

3858.35

Monoisotopic Mass

3855.50

Isoelectric Point

10.68

Extinction Coefficient [280nm]

8480.00

Activity

Kd: Voltage-Gated Sodium Channels

Target	Organism		Kd		Ref
Na_v1.2	R. norvegicus	(Expressed in X. laevis oocytes)	0.0034 Î¼M	+/-0.0013	Gajewiak,J. et al. (2014)
Na_v1.6	M. musculus	(With β3 subunit. Expressed in X. laevis oocytes.)	0.055 Î¼M	+/-0.015	Wilson,M.J. et al. (2015)
		(With β1 subunit. Expressed in X. laevis oocytes.)	0.029 Î¼M	+/-0.016	Wilson,M.J. et al. (2015)
		(Expressed in X. laevis oocytes.)	0.06 Î¼M	+/-0.023	Wilson,M.J. et al. (2015)
Na_v1.7	R. norvegicus	(Expressed in X. laevis oocytes)	0.013 Î¼M	+/-0.006	Gajewiak,J. et al. (2014)

References

Gajewiak,J., Azam,L., Imperial,J., Walewska,A., Green,B.R., Bandyopadhyay,P.K., Raghuraman,S., Ueberheide,B., Bern,M., Zhou,H.M., Minassian,N.A., Hagan,R.H., Flinspach,M., Liu,Y., Bulaj,G., Wickenden,A.D., Olivera,B.M., Yoshikami,D. and Zhang,M.M. (2014) A disulfide tether stabilizes the block of sodium channels by the conotoxin μO§-GVIIJ. Proc. Natl. Acad. Sci. U.S.A. 111:2758-2763

Wilson,M.J., Zhang,M.M., Gajewiak,J., Azam,L., Rivier,J.E., Olivera,B.M. and Yoshikami,D. (2015) α- and β-subunit composition of voltage-gated sodium channels investigated with μ-conotoxins and the recently discovered μO§-conotoxin GVIIJ. J Neurophysiol 113:2289-2301

Internal links

Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 28 March 2023