ViIA (P06858) Protein Card

General Information

Name	ViIA
Alternative name(s)	VilA-II
Organism	Conus virgo
Organism region	Indo-Pacific
Organism diet	vermivorous
Protein Type	Wild type
Protein precursor	VilA precursor (6859)
Notes	Only four alpha ono toxins belonging to 4/6 subgroup, including TxID, AuIB, Bu22 and TiLA, have been reported. VilA is the first 4/6 alpha-conotoxin that selectively inhibits nAChR a3b2 subtype ( Li et al. 2015)

Classification

Conopeptide class	conotoxin
Gene superfamily	A superfamily
Cysteine framework	I
Pharmacological family	alpha conotoxin

Sequence

RDCCSNPPCAHNNPDC(nh2)
Modified residues	position symbol name 17 nh2 C-term amidation
Sequence evidence	protein level
Average Mass	1740.87
Monoisotopic Mass	1739.60
Isoelectric Point	5.80
Extinction Coefficient [280nm]	NA

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism		IC50		Agonist	Ref
α2β2	R. norvegicus	(No SD or higher and lower brackets provided)	>10 uM		ACh (100 uM)	Li,L. et al. (2015)
α2β4	R. norvegicus	(No SD or higher and lower brackets provided)	>10 uM		ACh (100 uM)	Li,L. et al. (2015)
α3β2	R. norvegicus		845.5 nM		ACh (100 uM)	Li,L. et al. (2015)
α3β4	R. norvegicus	(No SD or higher and lower brackets provided)	>10 uM		ACh (100 uM)	Li,L. et al. (2015)
α4β2	R. norvegicus	(No SD or higher and lower brackets provided)	>10 uM		ACh (100 uM)	Li,L. et al. (2015)
α4β4	R. norvegicus	(No SD or higher and lower brackets provided)	>10 uM		ACh (100 uM)	Li,L. et al. (2015)
α7	R. norvegicus	(No SD or higher and lower brackets provided)	>10 uM		ACh (200 uM)	Li,L. et al. (2015)
α9α10	R. norvegicus	(No SD or higher and lower brackets provided)	>10 uM		ACh (30 uM)	Li,L. et al. (2015)

Synthetic variants

ViIA[R1A]	ADCCSNPPCAHNNPDC(nh2)
VilA deamidated	RDCCSNPPCAHNNPD(nh2)
VilA[D2A]	RACCSNPPCAHNNPDC(nh2)
VilA[H11A]	RDCCSNPPCAANNPDC(nh2)
VilA[ins15_L]	RDCCSNPPCAHNNPDLC(nh2)

References

Li,L., Liu,N., Ding,R., Wang,S., Liu,Z., Li,H., Zheng,X. and Dai,Q. (2015) A novel 4/6-type alpha-conotoxin ViIA selectively inhibits nAchR α3β2 subtype. Acta Biochim. Biophys. Sin. (Shanghai) 47:1023-1028

Internal links

Protein Precursor	VilA precursor (6859)
Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Thursday 1 June 2023