PeIA[E14(Gla)] (P08972) Protein Card

General Information

Name	PeIA[E14(Gla)] (named by ConoServer)
Alternative name(s)	[Gla14]PeIA
Organism	synthetic construct
Protein Type	Synthetic
Parent	PeIA

Classification

Conopeptide class	conotoxin
Gene superfamily
Cysteine framework	I
Pharmacological family	alpha conotoxin

Sequence

GCCSHPACSVNHP(Gla)LC(nh2)
Modified residues	position symbol name 14 Gla Gamma carboxylic glutamic acid 17 nh2 C-term amidation
Sequence evidence	Unknown
Average Mass	1695.87
Monoisotopic Mass	1694.60
Isoelectric Point	6.79
Extinction Coefficient [280nm]	NA

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		Agonist	Ref
α3β2	R. norvegicus	16.6 nM	[15.1-18.4]	Ach (100 uM)	Hone,A.J. et al. (2019)
α6/α3β2β3	R. norvegicus	28.5 nM	[25.7-31.6]	Ach (100 uM)	Hone,A.J. et al. (2019)
α6/α3β4	H. sapiens	18.2 nM	[14.9-24.4]	300 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	430 nM	[374-495]	300 uM ACh	Hone,A.J. et al. (2021)

References

Hone,A.J., Fisher,F., Christensen,S., Gajewiak,J., Larkin,D., Whiteaker,P. and McIntosh,J.M. (2019) PeIA-5466: A Novel Peptide Antagonist Containing Non-natural Amino Acids That Selectively Targets α3β2 Nicotinic Acetylcholine Receptors J. Med. Chem. 62:6262-6275

Hone,A.J., Kaas,Q., Kearns,I., Hararah,F., Gajewiak,J., Christensen,S., Craik,D.J. and McIntosh,J.M. (2021) Computational and Functional Mapping of Human and Rat α6β4 Nicotinic Acetylcholine Receptors Reveals Species-Specific Ligand-Binding Motifs. J Med Chem 64:1685-1700

Internal links

Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Monday 5 June 2023