RegIIA[N9A] (P09050) Protein Card

General Information

Name	RegIIA[N9A]
Organism	synthetic construct
Protein Type	Synthetic
Parent	RegIIA

Classification

Conopeptide class	conotoxin
Gene superfamily
Cysteine framework	I
Pharmacological family	alpha conotoxin

Sequence

GCCSHPACAVNNPHIC(nh2)
Modified residues	position symbol name 17 nh2 C-term amidation
Average Mass	1620.85
Monoisotopic Mass	1619.62
Isoelectric Point	8.42
Extinction Coefficient [280nm]	NA

Activity

Percentage inhibition: Nicotinic acetylcholine receptors

Target	Organism	% inhibition		Concentration	Agonist	Ref
α3β2	R. norvegicus	95		300 nM	ACh (50 uM)	Kompella,S.N. et al. (2015)
α3β4	R. norvegicus	5		300 nM	ACh (50 uM)	Kompella,S.N. et al. (2015)
α7	R. norvegicus	0		300 nM	ACh (200 uM)	Kompella,S.N. et al. (2015)

References

Kompella,S.N., Hung,A., Clark,R.J., Marí,F. and Adams,D.J. (2015) Alanine scan of α-conotoxin RegIIA reveals a selective α3β4 nicotinic acetylcholine receptor antagonist J. Biol. Chem. 290:1039-1048

Internal links

Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Saturday 1 April 2023