RegIIA[N11A,N12A] (P09057) Protein Card

General Information
Name RegIIA[N11A,N12A]
Organism synthetic construct
Protein Type Synthetic
Parent RegIIA

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSHPACNVAAPHIC(nh2)
Modified residues
positionsymbolname
17nh2C-term amidation
Sequence evidence protein level
Average Mass 1577.83
Monoisotopic Mass 1576.61
Isoelectric Point 8.42
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α3β2H. sapiens0 nMACh (100 uM)Kompella,S.N. et al. (2015)
R. norvegicus9.87 uM[7.9-12.4]-1.5ACh (50 uM)Kompella,S.N. et al. (2015)
α3β4H. sapiens259.4 nM[224.4-299.9]1.8ACh (50 uM)Kompella,S.N. et al. (2015)
R. norvegicus370 nM[309-442.3]-1.7ACh (50 uM)Kompella,S.N. et al. (2015)
α6/α3β4β3R. norvegicus3.4 uM[7.7-27]-1.1ACh (50 uM)Kompella,S.N. et al. (2015)
α7R. norvegicus21.5 uM[17.1-27]-3.1ACh (200 uM)Kompella,S.N. et al. (2015)

References
Kompella,S.N., Hung,A., Clark,R.J., Marí,F. and Adams,D.J. (2015) Alanine scan of α-conotoxin RegIIA reveals a selective α3β4 nicotinic acetylcholine receptor antagonist J. Biol. Chem. 290:1039-1048
Kompella,S.N., Cuny,H., Hung,A. and Adams,D.J. (2015) Molecular Basis for Differential Sensitivity of α-Conotoxin RegIIA at Rat and Human Neuronal Nicotinic Acetylcholine Receptors Mol. Pharmacol. 88:993-1001

Internal links
Nucleic acids
Structure

External links

Tools