PeIA[A7V, S9R, V10A, N11R, P13R, E14A] (P09953) Protein Card

General Information

Name	PeIA[A7V, S9R, V10A, N11R, P13R, E14A] (named by ConoServer)
Alternative name(s)	PeIA-5712
Organism	synthetic construct
Protein Type	Synthetic
Parent	PeIA

Classification

Conopeptide class	conotoxin
Gene superfamily
Cysteine framework	I
Pharmacological family	alpha conotoxin

Sequence

GCCSHPVCRARHRALC(nh2)
Modified residues	position symbol name 17 nh2 C-term amidation
Average Mass	1764.09
Monoisotopic Mass	1762.78
Isoelectric Point	12.80
Extinction Coefficient [280nm]	NA

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		Agonist	Ref
α1β1δε	H. sapiens	131 nM	[96.4-178]	100 uM ACh	Hone,A.J. et al. (2021)
α2β2	H. sapiens	>10000 nM		100 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	>10000 nM		100 uM ACh	Hone,A.J. et al. (2021)
α2β4	H. sapiens	>10000 nM		300 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	>10000 nM		300 uM ACh	Hone,A.J. et al. (2021)
α3β2	H. sapiens	12200 nM	[10710-13910]	100 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	9505 nM	[7331-12320]	100 uM ACh	Hone,A.J. et al. (2021)
α3β4	H. sapiens	9064 nM	[6061-13660]	300 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	>10000 nM		300 uM ACh	Hone,A.J. et al. (2021)
α4β2	H. sapiens	>10000 nM		100 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	>10000 nM		100 uM ACh	Hone,A.J. et al. (2021)
α4β4	H. sapiens	>10000 nM		300 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	>10000 nM		300 uM ACh	Hone,A.J. et al. (2021)
α6/α3β2β3	H. sapiens	5.55 nM	[4.17-7.39]	100 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	3.14 nM	[2.59-3.81]	100 uM ACh	Hone,A.J. et al. (2021)
α6/α3β4	H. sapiens	853 nM	[682-1065]	300 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	>10000 nM		300 uM ACh	Hone,A.J. et al. (2021)
α7	H. sapiens	5556 nM	[4544-6793]	300 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	3942 nM	[3209-4841]	300 uM ACh	Hone,A.J. et al. (2021)
α9α10	H. sapiens	576 nM	[433-768]	100 uM ACh	Hone,A.J. et al. (2021)
	R. norvegicus	667 nM	[539-825]	100 uM ACh	Hone,A.J. et al. (2021)

References

Hone,A.J., Kaas,Q., Kearns,I., Hararah,F., Gajewiak,J., Christensen,S., Craik,D.J. and McIntosh,J.M. (2021) Computational and Functional Mapping of Human and Rat α6β4 Nicotinic Acetylcholine Receptors Reveals Species-Specific Ligand-Binding Motifs. J Med Chem 64:1685-1700

Internal links

Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Monday 5 June 2023